Effect of flax seeds, pumpkin seeds and sesame seeds on calcium and phosphorus status of rats suffering from osteoporosis

This study was carried out to determine effect of two levels from flaxseeds, pumpkin seeds, sesame seeds and their combination on calcium and phosphorus status of rats suffering from osteoporosis. Sixty female rats [Sprague Dawley Strain] weighting an average of [200 +/- 10g] used in this study. The rats were divided into two main groups. The first main group [6 rats] was fed on basal diet as a [control negative group].The second main group [54 rats] fed on diet containing 100 mg [prednisone acetate] as source of glucocorticoid GC / kg diet to induce osteoporosis for two weeks. Then the second main group divided into nine subgroups [n=8 each] as follows: subgroup [1] fed on basal diet as a [control positive group], subgroups [2 and 3] fed on prednisone acetate diets containing [5% and 10%] flax seeds. Subgroups [4 and 5] fed on prednisone acetate diets containing [5% and 10%] pumpkin seeds. Subgroups [6 and 7] fed on prednisone acetate diets containing [5% and 10%] sesame seeds. Subgroups [8 and 9] fed on prednisone acetate diets containing [5% and 10%] combination of flax seeds, pumpkin seeds and sesame seeds. The results revealed that, food intake, body weight gain%, serum [calcium and phosphorus], femur bone [calcium and phosphorus], bone mineral density BMD and bone mineral concentration BMC of all treated groups improved than that of the positive control group, especially the osteoporotic group fed on diet containing 10% combination of [flaxseeds, pumpkin seeds and sesame seeds]. The Pathological examination of bone confirmed this results

Effect of flax seeds, pumpkin seeds and sesame seedson liver enzymes and kidney functions of rats suffering from osteoporosis

This study was carried out to investigate the effect of two levels from flax seeds, pumpkin seeds, sesame seeds and combination on liver enzymes and kidney functions of rats suffering from osteoporosis. Sixty female rats [Sprague dawley strain] weighting an average of [200 +/- 10g] used in this study. The rats were divided into two main groups. The first main group [6 rats] was fed on basal diet as a [control negative group].The second main group [54 rats] fed on diet containing 100 mg [prednislone acetate] as source of Glucocorticoid GC/kg diet to induce osteoporosis for two weeks. Then the second main group divided into nine subgroups [n=6 each] as follows: subgroup [1] fed on prednislone acetate diet as a [control positive group], subgroups [2 and3] fed on prednisone acetate diets containing [5% and 10%] flax seeds. Subgroups [4 and 5] fed on prednisone acetate diet containing [5% and 10%] pumpkin seeds, subgroups [6 and 7] fed on prednisone acetate diets containing [5% and 10%] sesame seeds. subgroups [8 and 9] fed on prednisone acetate diet containing [5% and 10%] combination of flax seeds, pumpkin seeds and sesame seeds. The results revealed that, food intake, body weight gain%, kidney functions [uric acid, urea nitrogen and creatinine] and liver enzymes [AST, ALT and ALP] of all treated groups improved than that of the positive control group, especially the osteoporosis group fed on diet containing 10% combination of [flax seeds, pumpkin seeds and sesame seeds]. The pathological examination of liver confirmed these results

Efeito da farinha de semente de abóbora (Cucurbita maxima, L) sobre o metabolismo glicídico e lipídico em ratos / The pumpkin (Cucurbita maxima, L) seed flour effect on the rat glucose and lipid metabolism

OBJETIVO: O objetivo desta pesquisa foi avaliar o efeito da farinha de semente de abóbora (Cucurbita maxima, L.) sobre o metabolismo glicídico e lipídico em ratos. MÉTODOS: Vinte ratos Wistar machos recém-desmamados, distribuídos em quatro grupos, receberam por 10 dias, rações controle e experimental com farinhas de semente de abóbora integral, peneirada ou residual substituindo 30 por cento do valor total de amido e dextrina da dieta controle. As dietas foram isocalóricas. Foram determinados os macronutrientes e a fibra insolúvel nas farinhas. As dietas tiveram a composição química calculada a partir dos dados dos rótulos dos produtos, da tabela de composição de alimentos e da análise química das farinhas de semente de abóbora. O peso corporal e a ingestão dos animais foram tomados a cada 48 horas. O sangue, coletado por punção cardíaca, teve os níveis de triacilgliceróis, colesterol e glicose analisados por métodos enzimáticos. RESULTADOS: As farinhas de semente de abóbora foram boas fontes de proteínas, lipídeos e, especialmente, fibras alimentares. Os animais tiveram ganho ponderal e ingestão semelhante (p>0,05). Os níveis de glicose e triacilgliceróis foram reduzidos significantemente para os grupos que receberam dietas com farinhas de semente de abóbora integral e peneirada. CONCLUSÃO: Frente às suas propriedades química e funcional, concluiu-se que a farinha de semente de abóbora interferiu no metabolismo do rato diminuindo significantemente os níveis de glicose e triacilgliceróis séricos.

OBJECTIVE: The aim of the present research was to evaluate the pumpkin (Cucurbita maxima, L.) seed flour effect on the rat glucose and lipid metabolism. METHODS: Twenty recent weaned male Wistar rats, divided in four groups, received for 10 days control and experimental diets containing whole, sifted and residual pumpkin seed flour on the rate of 30 percent of the total starch and dextrin in the control diet. All diets were isocaloric. Macronutrients and insoluble fiber were determined in the flours. All diets had their chemical composition calculated based on data from product labels, of food composition table and of the chemical analysis of the pumpkin seed flours. The animalsï growth and ingestion had been evaluated in 48 hours intervals. Blood samples drawn by cardiac puncture had their triacilglycerides, cholesterol and glucose levels measured by enzymatic methods. RESULTS: The pumpkin seed flour are good protein, lipids and, specially, dietary fiber sources.The animals' growth and food ingestion were similar along all the experiment (p>0.05). Glucose and triacilglycerides were significantly decreased in the groups taking diets with whole- and sifted-pumpkin seed flour respectively. CONCLUSION: Taking into account their properties chemistry and functional, it can be concluded that the pumpkin seed flour interfered in the rat metabolism decreasing significantly the serum glucose and triacilglycerides levels.